SubtiBank SubtiBank
yxeN [2019-09-30 10:26:42]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yxeN [2019-09-30 10:26:42]

putative S-(2-succino)cysteine [SW|ABC transporter] (permease)
locus
BSU_39490
pI
9.48
mw
24.86 kDa
protein length
224 aa Sequence Blast
gene length
675 bp Sequence Blast
function
uptake and utilization of S-(2-succino)cysteine
product
putative S-(2-succino)cysteine [SW|ABC transporter] (permease)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    4,058,791 → 4,059,465

    The protein

    Protein family

  • [SW|Binding-protein-dependent transport system permease family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|TcyL], [protein|9400DF25487461E3738DDDC72BDDB1589281409D|TcyB], [protein|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|YckA]

    • Structure

  • [PDB|4YMS] (amino acid transporter from Caldanaerobacter subterraneus, 39% identity) [pubmed|25848002]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B710 (yxeN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1709 NBRP B. subtilis, Japan]
  • 1A950 ( ''yxeN''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
  • BKE39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
  • BKK39490 (Δ[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC, downstream forward: _UP4_TACCGGACTTAGAGGTGAAA

    • References

  • 10746760,10092453,16513748,18763711,29626092,25848002